Increased Apoptosis but not PDX-1 Associated with Increase Glucose Calorie Interval

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Background: Several studies have conducted by in vitro as a model for assessing mechanisms to gain fluctuation of high blood glucose levels changes intermittently. However, the actual effects in the body (in vivo) might have different responses, since there are several signals mechanism in the body.

Aim: To determine the effect of calories on the diet of glucose intermittently on PDX-1, apoptosis, and Langerhans area.

Methods: Balb/c mice were divided into five groups. Control group were given a standard diet. The continuous group were given a standard diet and added 7.4% calorie continuously. The interval 1x, 2x, and 3x were given a standard diet and added 7.4% calorie 1x, 2x, and 3x/week. The 7.4% calorie addition was a glucose solution given by oral galvage and ad libitum for 8 weeks.

Results: There was a significantly difference on apoptosis density (p=0.043), but not in PDX-1. The Langerhans area of interval 2x and interval 3x showed a significant decrease than control group (p=0.048). Continuous insulin serum levels increased significantly compared to control group (p=0.04). In addition, the insulin serum of interval 1x and interval 3x were significantly lower than continuous group (p <0.05). Pre-post blood glucose levels on the treatment groups was significantly difference compared to control group (p=0.012) and the continuous group was the lowest.The ISI and HOMA-IR did not show any significant difference. However, HOMA-b% in the continuous group was significantly higher than in the other groups (p=0.009).

Conclusions: Continuous and 1-3x/week interval addition of 7.4% calories of glucose for 8 weeks indicate a compensation mechanism for maintaining homeostasis, such as increase insulin serum level and seem to initiate the changes of morphologic-biomolecular (PDX-1 and apoptosis in Langerhans).

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About the authors

Lilik Herawati

Faculty of Medicine, Universitas Airlangga

Author for correspondence.
ORCID iD: 0000-0002-8014-6952
ResearcherId: R-5238-2018
Mendeley Profile:

MD, Master of Health, Departement of Physiology

Indonesia, Jalan Mayjend Prof. Dr. Moestopo No. 47, Surabaya, Jawa Timur, 60131, Indonesia

Kristanti Wanito Wigati

ORCID iD: 0000-0002-8542-5934

Purwo Sri Rejeki

ORCID iD: 0000-0002-6285-4058

Widjiati Widjiati

ORCID iD: 0000-0002-8376-1176

Roedi Irawan

ORCID iD: 0000-0001-5999-4773


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